CONCANAVALIN A BINDING PROTEINS OF LYMPHOID CELL SURFACE
نویسندگان
چکیده
منابع مشابه
Concanavalin a Binding Proteins of Lymphoid Cell Surface
A Concanavalin A binding protein of chicken lymphoid cells was isolated by affinity chromatography of intact lymphoid cells. This protein with a mol wt of 160,000 daltons revealed two peptides (30,000 and 20,000 daltons) in SDS-acrylamide gel electrophoresis.
متن کاملBINDING SITES ON CONCANAVALIN A 399 Concanavalin
Equilibrium dialysis of concanavalin A against methyl c¢-D-mannopyranoside and methyl C¢-D-glucopyranoside conducted at 2 ° in the presence of I M NaC1 showed that concanavalin A is bivalent. A SCATCHARD plot of the data obtained gave straight lines for both sugars with observed association constants (K') of 1. 4. lO 4 1/mole for methyl ,¢-D-mannopyranoside and o.3" IO 4 1/mole for methyl ~¢-D-...
متن کاملIncrease in cell surface wheat germ agglutinin binding in a rat hepatoma cell line dRLa 74 treated with concanavalin A.
Wheat germ agglutinin binding to a rat hepatoma cell line dRLa 74 treated with concanavalin A was studied. It increased depending on the concanavalin A concentration in the culture medium. The cells exhibited about twofold increase in wheat germ agglutinin-binding when pretreated with 50 micrograms/ml of concanavalin A for 48 h. The wheat germ agglutinin binding sites were shown to be localized...
متن کاملIdentification of concanavalin A receptors and galactose-binding proteins in purified plasma membranes of Dictyostelium discoideum
Two techniques have been modified to provide simple means for the identification of molecules which bind concanavalin A (Con A). Crossed immunoelectrophoresis was altered by replacing antibody with Con A, and receptors were identified by the precipitin arcs which they produced. Con A, tagged with fluorescein isothiocyanate, was also diffused into prefixed sodium dodecyl sulfate (SDS)-polyacryla...
متن کاملBinding of concanavalin A to the surface of sea urchin eggs and its alteration upon fertilization.
The binding of concanavalin A (Con A) to Strongylocentrotus purpuratus eggs has been investigated using 125I-concanavalin A (125I-Con A). The lectin binds specifically to the egg surface and does not produce agglutination of the eggs. High 125I-Con A concentrations are necessary to saturate all of the surface binding sites. Scatchard plots of the binding data are biphasic and may be interpreted...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1974
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.140.2.597